Smooth muscle is one of the three different muscle types found in the body. It is involuntary, or contracts without conscious control, and unlike other muscle types, it lacks striations. Since it allows elasticity while maintaining an ability to contract, smooth muscle is suited for hollow organs in the body that need to expand and contract in order to hold and pass different substances, such as urine or blood. Its locations include blood vessels, lymphatic vessels, the gastrointestinal (GI) tract, the uterus, male and female reproductive tracts, the urinary bladder, the respiratory tract, the iris, the arrector pili, and the ciliary muscle.

Smooth muscle differs from the other two types of muscle, skeletal and cardiac, in form as well as function. Most strikingly different is its lack of striations, or stripes that run across the muscle cells, or fibers. In skeletal and cardiac muscles, the contractile components of the muscle fibers are organized into sarcomeres, and the end and middle of each sarcomere appear as stripes. Since smooth muscle is not organized into sarcomeres, it does not appear striped. Like cardiac muscle, but unlike skeletal muscle, smooth muscle cells typically have just one nucleus, typically located in the middle of the fiber. Under a microscope, one can tell smooth muscles apart from cardiac muscle by looking for intercalated discs, the dark stained lines between cells, which only occur in cardiac muscle.

The cells of this type of muscle are usually neatly grouped together in bundles or sheets and separated by connective tissue. The fusiform, or spindle-shaped, fibers contract by the action of actin filaments sliding over myosin filaments to shorten the length of the cell. The release of calcium triggers the contraction by phosphorylating, or adding phosphates (PO4) to the myosin. This differs from skeletal and cardiac muscle contractions, which occur when calcium binds to troponin, a component missing in smooth muscles. Smooth muscle contractions cannot be consciously controlled, like cardiac muscles, which allows for more rapid responses and continued function while the person is unconscious. They are instead controlled by the autonomic nervous system and neighboring cells that might send out chemical messengers to illicit contractions.

Smooth muscle can be either single-unit or multiunit. Each cell in multiunit muscles is innervated and contracts one by one, permitting more refined movement. This type of smooth muscle can be found in the iris, trachea, and aorta. Most smooth muscles in the body, however, are single-unit. In single-unit muscles, only one cell gets the signal to move, which causes the whole sheet or bundle to contract.