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What Is a Cloning Vector?

A cloning vector can be used to introduce foreign DNA into cells.
Specialized lab equipment is needed to work with cloning vectors.
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  • Written By: Mary McMahon
  • Edited By: O. Wallace
  • Last Modified Date: 24 October 2014
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A cloning vector is a segment of DNA which can be used as a carrier for foreign DNA, so that foreign DNA can be introduced into cells. Once the cloning vector is added to a cell, the foreign DNA can start to replicate itself, producing numerous copies of the foreign DNA which can be used for a number of purposes. Several labs commercially sell generic cloning vectors which can be used in research, and people can also craft their own for specific projects.

A number of organisms can be used as sources for cloning vectors. Some are created synthetically, as in the case of yeast artificial chromosomes and bacterial artificial chromosomes, while others are taken from bacteria and bacteriophages. In all cases, the vector needs to be genetically modified in order to accommodate the foreign DNA by creating an insertion site where the new DNA will fit.

A cloning vector must have an insertion site which can ideally accommodate many different strands of foreign DNA, allowing researchers to use the vector for different projects. It also needs a DNA sequence which will allow it to propagate itself once it is inserted into the target cell, and it needs a marker which researchers can use to find the foreign DNA once the cloning vector starts propagating. Antibiotic resistance is classically chosen as a marker so that cells can be introduced to an antibiotic which will kill cells without the foreign DNA, leaving the clones behind.

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Vectors for cloning can be used in many fields of scientific research. They can be used to duplicate a strand of DNA for the purpose of research and further investigation, as is done when people test DNA in a forensic lab or when people are looking for defective genes which may be responsible for genetic conditions. Cloning vectors are also used in genetic engineering, as they allow people to manipulate DNA and make numerous copies of the altered DNA.

Working with cloning vectors requires a lab with equipment which allows people to manipulate DNA. Using a stock cloning vector from a lab can cut down on the amount of time needed to develop a vector, but researchers still need to be able to select the strand of foreign DNA they wish to insert and integrate it into the cloning vector with the assistance of restriction enzymes which separate the strands of DNA in the vector to allow for the insertion or “ligation” of the new DNA.

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irontoenail
Post 2

@indigomoth - That's not quite right. A cloning vector isn't deactivated. You're thinking of another kind of cloning, where they insert DNA into an empty host cell with the intention of growing an embryo, I think.

I think the purpose of a cloning vector is to provide a space in a DNA sequence that is able to invade a cell and replicate, but not much else, so that you can stick whatever DNA you like in there and have it replicated.

So, you modify bacteria DNA, for example, to accept the bit of DNA you want copied, then insert the whole thing into a cell. The bacteria just gets on with copying itself like usual, except now it is copying your DNA along with it.

In fact, I think people should be more worried about the fact that antibiotic resistance is considered desirable for a cloning vector. That seems like a recipe for disaster if you happen to have one careless scientist...

indigomoth
Post 1

We learned about this in my biology classes. This is one aspect of the arguments against genetic engineering that is often used by protesters. Because, often a cloning vector is a virus or a bacteria cell. So it's easy to come up with a scare-mongering headline exclaiming that scientists are infecting animals or plants with new DNA or diseases or melding together bacteria and DNA from other creatures.

In reality the cloning vector cell is usually completely deactivated, and is no more than a package for the DNA. So, it could be any kind of cell, bacteria are just easy to grow and isolate.

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