Antagonistic muscles are muscles which work in opposition to each-other. For example, a person would use certain sets of muscles to open his hand and splay his fingers wide. In order to close the hand and make a fist, however, an antagonistic set of muscles would have to be used. Antagonistic muscles are important for balance, extending limbs, holding objects aloft, and contracting limbs.

Antagonistic muscles are necessary for the body to function properly. Skeletal muscles can only tighten (contract); in a sense, they can be compared to a rope tied around a rock that can only be pulled tight in one direction or allowed to go slack. In order for a body part to move in the opposite direction, the antagonistic muscle group must be used. Returning to the rock metaphor, another rope must be tied to the opposite side of the rock and pulled taught if the rock is to be moved in the opposite direction.

There are many antagonistic muscle groups in the body. The most famous of these pairings is the biceps (biceps brachii) and the triceps (triceps brachii) on the arm. Additional antagonistic muscles in general terms would be the chest and back of the torso, as well as the quadriceps and hamstrings of the leg.

Dynamic tension exercises like those made famous by Charles Atlas sometimes use antagonistic muscle groups. In one exercise, hands are clasped in front of the chest and both arms push against each-other. Slowly, the arms are allowed to move to the left, then slowly to the right. This exercise works antagonistic muscle groups, though on opposite arms at any given time.

Weightlifters often debate the best way to train antagonistic muscle groups. Some believe that antagonistic muscle groups should be worked on in the same day in order to make significant gains in limited amounts of time. Other weightlifters believe that simply doing exercises slowly will work both muscles in an antagonistic muscle group sufficiently; doing different exercises that individually target both muscles in the group will result in overtraining. Weightlifters from both schools of thought have done well in competition, leaving the question up for continued debate.